(a):

Plasmids encoding GEiGS solutions were transfected into iPSCs and analysed for B2M expression via cell surface staining at Day 3. The expression of the transfected solution was corrected against a red fluorescent reporter on the plasmid, and B2M expression was normalised to the sample transfected with a negative control solution.

(b):

Following GEiGS implementation via gene editing, clonal cell lines were genotyped to identify heterozygous and homozygous clones. Clones were expanded and analysed for B2M expression via cell surface staining.

(c):

Primary T-cells were edited via electroporation with CRISPR RNP-based reagents. Cells were genotyped and analysed for B2M expression via cell surface staining at Day 3.